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KMID : 0043319990220030288
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Archives of Pharmacal Research
1999 Volume.22 No. 3 p.288 ~ p.293
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The Application of Ion Chromatographic Method for Bioavailability and Stability Test of Iron Preparations
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Kim Young-Ok
Chung Hye-Joo
Kong Hak-Soo
Choi Dong-Woong
Choi Dae-Hyun
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Abstract
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Postabsorptive serum iron level was determined after oral administration of the compounds to human. In serum and whole blood, was measured by ion chromatography (IC) using a pyridine-2,6-dicarboxylic acid (PDCA) as an eluent. The serum sample solutions were pretreated with I N HCI and 50% TCA. The whole blood sample solutions were treated with 3 N HCI for 30 min at . The limit of detection (LOD) of the IC technique is forand 0.1 M for . The area under concentration (AUC) can be obtained by the above analytical condition. In addition, to compare the stability of to that of in pharamaceutical preparations, accelerated stability test was carried out. After storing the samples under , 75%RH in light-resistant container for various time intervals, the contents of iron of different valencies were determined separately by the IC technique and the change and/or the interchange of among those iron species in preparations was investigated. Iron raw materials are stable, but in source materials was slightly converted to by oxidation. in source raw materials and in raw materials are determined as impurities. Therefore, IC technique is found to be an appropriate method for comparative evaluation of dissimilar bioavailability of and , stability of and raw materials and preparations.
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KEYWORD
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Ion chromatography (IC), Bioavailability, Stability, AUC, LOD, $Fe^{2+}, Fe^{3+}$
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